1x click-it edu reaction buffer  (Thermo Fisher)

Journal: Developmental biology

Article Title: Gon4l/Udu Regulates Cardiomyocyte Proliferation and Maintenance of Ventricular Chamber Identity During Zebrafish Development

doi: 10.1016/j.ydbio.2020.03.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Reagent or resource Source Identifier Antibodies MF20 Developmental Studies Hybridoma Bank AB_2147781 S46 Developmental Studies Hybridoma Bank AB_528376 Mef2C Abcam Ab64644 Casp3 Cell Signaling P42574 Cat#: 9661S Bacterial and Virus Strains Biological Samples Chemicals, Peptides, and Recombinant Proteins o-Diansidine Sigma Cat#: D9143-5G Critical Commercial Assays EdU Click-It Reaction Buffer Thermo Fisher Scientific Cat#: C10340 ApopTag Peroxidase In Situ Apoptosis Detection Kit Millipore Sigma Cat#: S7100 Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains udu vu66 Williams et al., 2018 N/A Tg( cmlc2:GFP ) Huang et al., 2003 ZDB-ALT-060719-2 Tg( hsp70l:nkx2.5-eGFP ) George et al., 2015 ZDB-TGCONSTRCT-150527-1 nkx2.5 vu179 Targoff et al., 2013 ZDB-ALT-131212-1 t p53 zdf1 Berghmans et al., 2005 ZDB-ALT-050428-2 Oligonucleotides Primers for qRT-PCR: cnne1, cnne2, cnnd1, cnnd2a, cnnd2b, cnnd3, nkx2.5, nkx2.7, gapdh See supplemental Table S1 Liu et al., 2007 Targoff et al., 2008 N/A Primers for genotyping udu vu66 , Tg( hsp70l:nkx2.5-eGFP ), nkx2.5 vu179 , t p53 zdf1 See supplemental Table S2 Williams et al., 2018 Targoff et al., 2013 Berghmans et al., 2005 N/A Recombinant DNA Software and Algorithms Other Open in a separate window KEY RESOURCES TABLE.

Techniques: Virus, Recombinant, In Situ, Software